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2023, doi:10.3389/fendo.2023.1271521 This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)

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The cells from tumor tissue were stained with anti-CD3-PE, anti-CD4-APC, and anti-CD8-FITC antibodies for 30 min, and further analyzed the ratio of different types T cells by flow cytometry