PfGrx shares 39% sequence identity with human Grx1, including a CPYC motif and a semiconserved GGC motif, and is well characterized in vitro and in roGFP2 assays in yeast 14,26,30,35
Next, the microplate was placed in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 37 C, followed by horizontal shaking for 30 s and subsequent reading of absorbance at 570 nm every minute until the value of the most active sample surpassed that of the highest standard
A 200 mcg dose requires 0.2 mL, drawn to the 20-unit mark on a U-100 insulin syringe
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