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After purification, the protease is added to the eluted fractions to cleave the GST tag from the target protein
(Notably, the observational analysis found no increase in depression reports with tirzepatide , even though tirzepatide causes similar GI side effects and often even greater weight loss than semaglutide (eClinicalMedicine 2025)
They don't require heat shrink, as the plastic housing shrouds the terminals completely
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