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Snderhauf et al, 2020
Proteomics sample preparation Frozen tissue samples were homogenized in 0.1 M Tris-HCl, pH 7.6 and 2% SDC, heated for 5 min at 95 C and sonicated (Diagenode Bioruptor at high intensity, 15 30 s)
This activity could, at least in part, come from the translocation of DPP-4 activity from the microbiota and that even if the amount of enzyme were small, it may have an effect on the host's metabolism
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