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Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s

Cytosolic proteostasis relies heavily on chaperones, sequestration systems, and UPS-mediated degradation

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The mean percentage change in body weight from baseline to 68 weeks was 14.9% in the semaglutide group versus 2.4% in the placebo group