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The laccase activity was measured by adding 180 l of reaction mixture (100 mM citratephosphate buffer pH 4.0 and 1 mM ABTS) by using a Multidrop robot (Multidrop Combi, Thermo Scientific)

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Increased erythrocyte oxidative stress results in the opening of multiple ion channels, comprising the nonselective cation channels (NSCC), like sodium, potassium, and calcium channels [202, 404]