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This phenomenon often occurs within the first few weeks of starting therapy and tends to persist with ongoing use
However, these strategies often fail to offer sustainable solutions, as the body's powerful biological mechanisms fight to regain lost weight
Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h
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