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For the mitochondrial OCR assays, mitochondria were plated on Seahorse Biosciences 96-well culture plates (5 g of protein per well) and centrifuged for 20 min at 2,000 g at 4 C to adhere mitochondria to the base of wells
Residues forming the hydrophobic pocket are displayed in pink, the Phe residue that moves between the open and closed conformation is displayed in orange
Ipamorelin is a pentapeptide (five amino acids: Aib-His-D-2-Nal-D-Phe-Lys-NH2) with a molecular weight of 711.85 g/mol, meaning its three-dimensional structure is sensitive to mechanical stress, temperature fluctuation, and pH variance
Y., Chin Han Lew, C., Hill, A., Jeschke, M
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