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Experimentally, solute permeabilities are determined using a transwell assay from the concentrations in the apical (luminal, C L ) and basolateral (abluminal, C A ) compartments after time t using: where V A is the volume of the basolateral compartment (see Supporting Material for details)
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Luminescence measurements were performed using 475/30 nm and 535/30 nm filters with baseline measurements taken for ~3 min before addition of vehicle or peptide and reading resumed for a further 10 min
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