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The pipette (extracellular) solution contained 140 mM KCl, 2.6 mM CaCl 2 , 1 mM NaCl, 1.2 mM MgCl 2 , and 10 mM HEPES (pH 7.4, by KOH), while the bath (cytosolic) solution contained 130 mM NaCl, 5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES (pH 7.4, by NaOH) supplemented with desired concentrations of glucose and NMDG to bring the final osmolarity of 300 mOsm