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Extracellular Rna as a kind of communication molecule and emerging cancer biomarker
After incubation with primary antibodies, the membranes were washed in TBS with 0.05% Tween (TBST) three times for 15 minutes and incubated with anti-goat, anti-rabbit, or anti-mouse HRP-linked secondary antibody at concentration 1:7,500 for 60 minutes at room temperature with gentle agitation
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