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People taking high-dose B12 supplements (1,000 to 5,000 mcg daily), receiving B12 injections (cyanocobalamin or methylcobalamin), or consuming heavily fortified foods can easily push their serum levels above 900 pg/mL

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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BLC1

They need to be as safe as possible as they actually prevent (they are prophylactic ) rather than treat diseases and are often used for children