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S961 treatment in vivo Vehicle (PBS) or 20 nmol S961 (Phoenix Pharmaceuticals) with scrambled MOTS-c or MOTS-c (0.5 mg kg 1 per day) was loaded into Alzet osmotic pump 2001 or 2004 and implanted subcutaneously on the back of the 28-week-old C57BL/6 mice 1,24,25 and changed weekly for a total of 2 weeks for 2001
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