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10.3892/ijmm.2021.5039 103 ShulmanG
Total RNA was extracted using TRIzol (Life Technology), reverse transcribed into cDNA using the GoScriptTM Reverse Transcription System (Promega) and analyzed by real-time qPCR on a BIO-RAD Real-time PCR machine using iTaq Universal SYBR Green Super mix (BioRad)
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The thyroid gland is a butterfly-shaped organ that is located on the anterior (front) side of the neck
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