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For the nanoprecipitation method, PLGA polymer was dissolved in a suspension of DDA-GSH-AuNCs in dichloromethane (DCM) (after the phase transfer) and the resulting mixture was added drop wise into ethanol (non-solvent for PLGA and non-suspending medium for DDA-GSH-AuNCs) resulting in the formation of PLGA nanoparticles loaded with DDA-GSH-AuNCs (cartoon for the encapsulation process is sketched in Fig

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8-12 weeks 2 10 mL bottles 8 weeks: Uses 12 mL across the cycle