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The blood-brain barrier: physiology and strategies for drug delivery
Macrophages were induced from THP-1 cells and PBMCs using 10 nM phorbol-12-myristate-13-acetate (PMA), then induced into M1-like macrophages with lipopolysaccharide (1 g/mL) and IFN- (20 ng/mL) for 48 h, or induced into M2-like macrophages with IL-4 (20 ng/mL) for 48 h
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Gently swirl the vial without vigorous shaking to avoid damaging the peptide structure
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