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Men, largely because they tend to be heavier, usually need to drink more than most women to have the same BAC

After 3 washes with PBS, cells were incubated with Alexa fluor 488 or 555 secondary antibodies (1:1000) (Thermo Fischer Scientific) as detailed in Supplementary Table S3 for 1 h at room temperature

The difference between these gauges is the needle thickness