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Procedure Procedure I
After the amplification, a melting peak analysis with a temperature gradient from 72 C to 95 C was performed
Disturbed iron metabolism triggers the Fenton reaction and induces the accumulation of ROS, accumulation of lipid peroxides, and insufficient Glutathione Peroxidase 4 (GPX4) leading to the difficult conversion of lipid hydroperoxides are at the core of the ferroptosis induced by iron metabolism disorders
RAW264.7 and HEK293 cells were cultured in DMEM (Wisent) supplemented with 10% fetal bovine serum
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