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In our study, in order to obtain a more accurate threshold and minimize the subjective bias, the following procedures were strictly followed: (1) a black patch covered the eyes to avoid subjective influence from the participants, (2) the vibratory sensation probe was applied to contact with the penis with its own weight rather than hand pressure to avoid variations in pressure, (3) the vibratory stimuli were provided in an ascending manner until the first report of the sensation of vibration and then in a descending pattern from perception to no perception of vibration, (4) tests were repeated after a 5 minute break if the differences between two consecutive measurement for the same position were greater than 10%

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CYP3A4 is one of these, and is predominantly present in the intestine