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After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)

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[1] [7] These findings are not directly comparable to results from trials of semaglutide or tirzepatide, as no head-to-head trials have been conducted