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Protein extracts were separated by electrophoresis on 412% NuPAGE Bis-Tris Mini Gel and then transferred onto nitrocellulose membranes using iBlot transfer stacks (Life Technologies, Gaithersburg, MD, USA)
Early pharmacology work described it as a selective growth-hormone secretagogue, with GH release and little ACTH or cortisol signal in the foundational studies
The secondary evaluation outcomes were alterations in blood urea nitrogen (BUN), uric acid (UA), estimated glomerular filtration rate (eGFR), urinary AKI biomarkers, renal fibrosis biomarkers (transforming growth factor- 1 (TGF- 1 ), connective tissue growth factor (CTGF)), and traditional Chinese medicine (TCM) symptoms
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While prior literature cites a 510% defect rate among women with pregestational diabetes, this study did not report any significant pregnancy losses following the use of GLP-1RA (92)
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