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Fat under the eyes typically starts to disappear earlier here than from other spots on the face, so hollowness here often stands out on a young face, or is much worse here than other places on the aging face 3

Permeabilization and blocking was performed by adding PBS (Lonza) containing 0.1% saponin (Carl Roth) and 3% normal donkey serum (Abcam, Cambridge, UK) for 30 min at RT

Both peptides are supplied as 5 mg lyophilized powders, typically with purity 95%, verified through analytical methods such as HPLC and mass spectrometry

Effect of Physical Activity on Glutamine Metabolism. Current Opinion in Clinical Nutrition and Metabolic Care 13(1): 5864