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Patients should be aware that heavy, chronic ethanol use may counteract the therapeutic effects of vitamin B12
Brains were fixed, cryoprotected, and sectioned for immunofluorescent labeling using anti-Fos antibodies and fluorescent secondary antibodies
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This combination enables researchers to study complementary pathways: GHRH receptor activation plus selective GHS-R stimulation, with minimal off-target effects on prolactin, cortisol, or ACTH
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