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Reductions in muscle volume seem commensurate with what would be expected given the degree of weight loss achieved, and improvements in insulin sensitivity and muscle fat infiltration likely contribute to better muscle quality even when total volume decreases somewhat
This method, based on Pelser et al.s 1996 methodology with modifications, involved heating the sample with thiobarbituric acid (TBA) to form a purple complex whose intensity correlates with MDA quantity, measured at 532 nm with a spectrophotometer [19]
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