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Glutathione not only functions as the substrate for protein S-glutathionylation but can also reverse the process by reducing the cysteine in proteins to which glutathione is disulfide bonded forming oxidized glutathione, GSSG

in vitro Semaglutide is selected as the optimal once weekly candidate

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Intracellular GSH levels were assessed using ThiolTracker Violet (T10095, Thermo Fisher Scientific), and ROS levels were measured using DCFDA (HY-D0940, MedChemExpress)