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The activation of Akt results in skeletal muscle growth/maintenance since it controls the phosphorylation of a number of substrates involved in MPS including mTOR (and its downstream targets 4E-binding protein 1 (4E-BP1) and p70S6 kinase) and glycogen synthase kinase 3 (GSK3), as well as, the inhibition of protein degradation via the forkhead transcription factor (FOXO) pathway (Consitt et al., 2006)
This test helps in diagnosing trichotillomania by examining the hair follicles and surrounding skin tissue under a microscope to identify signs of damage from hair-pulling
Total RNA was extracted from with Trizol reagent (Beyotime, Shanghai, China) according to the manufacturers instructions
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