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To view a copy of this license, visit About this article Cite this article Franchina, D.G., Kurniawan, H., Grusdat, M

doi: 10.3389/fphar.2026.1761748 Received 05 December 2025 Revised 25 March 2026 Accepted 01 April 2026 Published 22 April 2026 Volume 17 - 2026 Edited by Ming Xu, Shimonoseki City University, Japan Reviewed by Gaetano De Siena, University of Florence, Italy Wei Guo, University of North Carolina at Greensboro, United States Updates Copyright 2026 Nandagopal and Manickam

These purified HBs demonstrated robust self-renewal capacity, expanding in a small-molecule-defined medium (ACDFSV) for over 20 passages without losing their proliferative capacity or characteristic morphology