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Understanding why refrigeration is necessary comes down to basic chemistry: proteins denature at higher temperatures

For the extraction of total RNA, tissue samples were pulverized using GenoGrinder (ColeParmer), then homogenized in Tri Reagent (Molecular Research Center or Ambion by Life Technologies) using a MagNa Lyser or TissueLyser II system (Qiagen)

Here's what can happen when we don't get enough magnesium: Oxidative stress and mitochondrial dysfunction, resulting in decreased ATP production

Additionally, the available clinical trials did not measure the biomarkers of oxidative damage, so its mechanism of action may not be indeed related to the antioxidant effects