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After full recovery, correct cannulation was tested by observing water drinking responsiveness after infusion of 1 l human angiotensin II (Sigma-Aldrich, A9525) at a concentration of 24 M in artificial cerebrospinal fluid (distilled water with 125 mM NaCl, 2.5 mM KCl, 2.6 mM NaHCO 3 , 1.25 mM NaH 2 PO 4 2H 2 O, 25 mM d-glucose monohydrate, 1 mM MgCl 2 , 2 mM CaCl 2 )

It can also increase insulin resistance, making it harder to lose weight and easier to gain weight

Discuss any possible side effects and how long you should expect them to last

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