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Bring the barrel to eye level, line the plunger tip up with the 20 mark, and check it before the needle touches skin
2.7 western blotting Total proteins were extracted from liver tissues using RIPA buffer (Beyotime Biotech, China) supplemented with 1 mM PMSF (Beyotime Biotech, China)
Materials and Methods Animals and Dietary Intervention Male Wistar rats (250280 g) were individually housed in a temperature (2123C) and light-controlled room (lights on 07001900)
Under sustained reductions in nutrient inflow, metabolic reserve may narrow as reduced substrate availability limits mitochondrial redox regeneration and constrains the capacity to match oxidative flux with regenerative turnover
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